Effect of pyriproxyfen on neural cell differentiation in the forebrain and midbrain of Gallus domesticus E10 embryos. (A–D) Cross-sections and graphs of vimentin-positive cells and (H–J) NeuN-positive cells (n = 3 brains/PPF concentration). E, F, K, and L, Relative transcript levels of vim, gfap, fox3, and tubb3 (n = 9 brains/PPF concentration). M and N, Cross-sections showing neuronal dendritic branches. O and P, Area occupied by neurons, (Q and R) length of dendritic branches, and (S and T) number of bifurcation dendrites (n = 6 brains/PPF concentration). In cross-sections, black arrowhead points for vimentin-positive cells (A, B), NeuN-positive cells (G, H), and neuronal dendritic branches (M, N). Scale bars = 20 μm. Mean ± standard error of the mean (SEM). * p < .05, ** p < .01, *** p < .001, **** p < .0001.