On-target and off-target SNPs in mitochondrial genomes and chloroplast genomes in 6 representative T₂ plants (3 progenies of 2 T₁ lines of atp6-2-2 A) and 16S rRNA-1 B). None of these plants contained mTALEAD genes. The x- and y-axes show the positions and frequencies of the mutated SNPs that differed by at least 5% from the reference genomes BK010421.1 (mitochondria) and AP000423.1 (chloroplast). AF was calculated as AF_mu−AF_WT, where AF_mu is an AF of a SNP of each mutant and AF_WT is the average of the AFs of the same SNP of 3 WT plants.