ZmWUS1-B-edited alleles do not increase kernel row number but enhance maize prolificacy. A and B) Comparison of inflorescence meristem size (in μm; y axis) and kernel row number of WT and heterozygous ▵113 and ▵33 alleles. For the quantification of inflorescence meristem sizes, n = 8 in +/▵113 plants and WT siblings; n = 12 in +/▵33 plants, and n = 10 in WT siblings. For the quantification of kernel row number, n = 43 in +/▵113 allele plants and 65 in WT siblings, 28 in +/▵33 allele plants, and 44 in WT siblings. C) The heterozygous ▵33 allele showed increased prolificacy. Scale bars 10 cm. D) Quantification of ear numbers of the primary lateral branches in heterozygous ▵113 and ▵33 alleles. n = 56 in +/▵113 allele plants and 65 in WT siblings, 44 in +/▵33 allele plants, and 63 in WT siblings. Student's t tests P-value annotation legend: ns, P > 0.05; **0.001 < P < 0.01; ****P < 0.0001; error bars indicate 95% confidence interval.