The overall flowchart of Enhancer-MDLF. (A) Sequence encoding: Enhancer-MDLF utilizes two distinct sequence encoding schemes, namely dna2vec and Motif frequency. (B) Model construction: Enhancer-MDLF is structured by integrating two pivotal modules: the dna2vec module and the motif module. (C) Model evaluation. Enhancer-MDLF undergoes thorough evaluation across multiple dimensions to assess its performance. (D) Motif analysis: Utilizing the SHAP framework, we conduct an in-depth analysis of the important features identified by Enhancer-MDLF.