Effects of CLPr supplementation on the lifespan of wild-type N2 worms. Survival curves for N2 worms in the presence (solid lines) and absence (control, dotted lines) of 10 µg/mL CLPr. (A) Survival curves of N2 worms fed live or heat-killed Escherichia coli OP50 bacteria at 20°C. (B) Survival curves of N2 worms at 25°C, 20°C, and 15°C fed live bacteria. (C) Survival curves of N2 worms in the absence (thin lines) and presence (thick lines) of 40 µM FUdR at 25°C. The results from multiple experiments were combined to form a single curve for each experiment. Statistical evaluation was performed using the log-rank test. Asterisks (***) indicate p < .001, and N.S. indicates no significant difference. The number of worms, mean lifespan, change in mean lifespan, and statistical significance of each trial are shown in Supplementary Table S1, along with the combined data.