![Microbial community successions on brown macroalgal leachate; (A) experimental setup for studying the succession of natural microbial communities on leachate of the brown seaweed F. vesiculosus (FL); left: map of Nahant [58] showing the sampling locations of the seawater and sediment communities used as inocula (scale: 1:10 000); right: schematic of the self-assembly process on FL; inoculant communities were grown in FL batch media for 216 h, and at each time point between 6 and 216 h, cell count and DOC measurements were taken; community composition was determined in the inocula and at each time point by 16S rRNA gene tag sequencing; ~30–40 isolates were collected from each replicate community at each time point; (B) average trajectory of total bacterial abundances (as cell density) and the change in concentration of DOC relative to t = 6 h (negative values = decrease of DOC) in the seawater- and sediment-inoculated communities over time; error bars are standard deviations (n = 3); (C) community composition over time shown as the relative abundances of taxa averaged over three replicates from seawater- and sediment-inoculated communities; the top 40 most abundant taxa (amplified sequence variants, ASVs) are shown in color; the sum of other ASVs is shown in gray; the community composition changes from 0 to 6 h in the seawater-inoculated communities are in a separate panel on the left to emphasize the change from the inoculum to the first bloom; (D) distribution of the COM, $\sum [\mathrm{Cell}\ \mathrm{Density}\times \log (\mathrm{time})]/\sum \mathrm{Cell}\ \mathrm{Density}$) for the absolute abundance of individual taxa in the seawater- (blue, left) and sediment- (brown, right) inoculated communities; distributions are represented as both histograms (colored bars) and kernel density estimations (black lines); absolute abundances are calculated as the product of community cell density in (B) with relative abundances in (C); gray lines at the first trough of density plots represents the cutoff between early and late growers in the successions; colored dots represent the five selected representative ASVs from early and late bloomers, respectively.](https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/ismej/18/1/10.1093_ismejo_wrad013/3/wrad013f1.jpeg?Expires=1748983278&Signature=gy4QcNzj~~i4EtIOb7~612sVNWO9WAsxgQt7IR3ZYvVWInd03sQTTzPMXmFKbMw8oohOEpzHzNd3ENmlwiZBcPkJsgr9k3n7eL-~wHZI8FVbz9Xs8epvhUV9uVxDQVG4ikFqIzd~0u8wC2wQCsvrYFijKPHG5kdKr0k6vRpszCAuqgzLKPrI2XXTV8jLX83GSIeEu~Vek~OM3PlSktxBjb3PfYA4jMMMqMCJDPwDmcRRh36J-G-7qs3hmZTaYjip6UkZvJqiQEQTQbp8sfFPJc-1sqlCEIjQLHM~VYsyIE121ZLHGNUxX~CEnBbMED2ueyFL6NXBFTC~Obm-PAhU5Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Microbial community successions on brown macroalgal leachate; (A) experimental setup for studying the succession of natural microbial communities on leachate of the brown seaweed F. vesiculosus (FL); left: map of Nahant [58] showing the sampling locations of the seawater and sediment communities used as inocula (scale: 1:10 000); right: schematic of the self-assembly process on FL; inoculant communities were grown in FL batch media for 216 h, and at each time point between 6 and 216 h, cell count and DOC measurements were taken; community composition was determined in the inocula and at each time point by 16S rRNA gene tag sequencing; ~30–40 isolates were collected from each replicate community at each time point; (B) average trajectory of total bacterial abundances (as cell density) and the change in concentration of DOC relative to t = 6 h (negative values = decrease of DOC) in the seawater- and sediment-inoculated communities over time; error bars are standard deviations (n = 3); (C) community composition over time shown as the relative abundances of taxa averaged over three replicates from seawater- and sediment-inoculated communities; the top 40 most abundant taxa (amplified sequence variants, ASVs) are shown in color; the sum of other ASVs is shown in gray; the community composition changes from 0 to 6 h in the seawater-inoculated communities are in a separate panel on the left to emphasize the change from the inoculum to the first bloom; (D) distribution of the COM, |$\sum [\mathrm{Cell}\ \mathrm{Density}\times \log (\mathrm{time})]/\sum \mathrm{Cell}\ \mathrm{Density}$|) for the absolute abundance of individual taxa in the seawater- (blue, left) and sediment- (brown, right) inoculated communities; distributions are represented as both histograms (colored bars) and kernel density estimations (black lines); absolute abundances are calculated as the product of community cell density in (B) with relative abundances in (C); gray lines at the first trough of density plots represents the cutoff between early and late growers in the successions; colored dots represent the five selected representative ASVs from early and late bloomers, respectively.
