CG9035 mediates the glycosylation pathway to regulate lipoprotein maturation. (a) PCA of the metabolic data in the white RNAi and the dAcslRNAi group (n = 4). (b) Volcano plot illustrating DEPs, defined by fold change ≥ 1.2 and P < 0.05 in fat body-specific dAcsl knockdown flies compared to the controls; four biological replicates per group, and each replicate comprised 20 fat bodies. (c and d) Quantified levels of glycosylation substrates UDP-glucose (c) and UDP-GlcNAc (d). (e) Metabolic pathway enrichment analysis results (top 25) (MetaboAnalyst). (f) Green fluorescence showing BODIPY-labeled lipid droplets in cg > CG9035 RNAi and cg > white RNAi flies. (g) Green fluorescence showing BODIPY-labeled neutral lipids in cg > white RNAi, cg > dAcsl RNAi, and cg > dAcsl RNAi; CG9035^OE flies. (h) Statistical results showing cg > dAcsl RNAi; CG9035^OE neutral lipids area compared with cg > white RNAi and cg > dAcsl RNAi groups (n = 20). Numbers in the figure represent P values. Scale bar = 20 μm.