Gut neutral lipid accumulation deposition in dAcsl RNAi flies was restored by apoLpp overexpression. (a) Volcano plot illustrating DEPs as defined by P < 0.05 from Welch’s t-test in dAcsl RNAi flies compared to white RNAi flies. Proteins increased in dAcsl RNAi flies are indicated by red dots, while those reduced compared with the controls are represented by green dots. Each set of three biological replicates contains 20 fat bodies per replicate. (b and c) Normalized protein intensity of apoLpp (b) and dAcsl (c) (n = 3). (d) Green fluorescence showing BODIPY-labeled lipid droplets in the fat bodies of ApoLpp RNAi and LTP RNAi flies. Scale bar = 20 μm. (e) Green fluorescent showing BODIPY-labeled neutral lipids in the fat bodies of cg > dAcsl RNAi; apoLpp^OE and cg > dAcsl RNAi flies. Scale bar = 20 μm. (f) Statistical results of cg > dAcsl RNAi; apoLpp^OE and cg > dAcsl RNAi flies (n = 8). Numbers in the figure represent P values.