Interaction between ILIs and OsbHLH157/OsbHLH158. A) Interaction between ILIs and OsbHLH157/OsbHLH158 in yeast 2-hybrid assay. SD/-TL, selective medium lacking Trp and Leu. SD/-TLHA, lacking Trp, Leu, His, and Ade. X-α-Gal (5-bromo-4-chloro-3-indolyl-α-d-galactopyranoside) was added to indicate the β-galactosidase activity of transformed yeast cells. Yeast transformations of the empty prey (AD) and OsbHLH157/OsbHLH158-BD were used as negative control. B) Interaction between ILIs and OsbHLH157/OsbHLH158 using BiFC assay. Bars, 20 μm. C) Split-LUC complementation analysis of the interaction between ILIs and OsbHLH157/OsbHLH158.