CaCl₂ treatment of pregerminating seedlings results in radicle-focused expression of HKT1;1. A) GUS staining of HKT1;1pro:GUS-harboring pregerminating seedlings treated with single or combined chemicals as indicated (NaCl, 200 mm; CaCl₂, 10 mm) for 16 h prior to ejection, staining, and imaging. Bar = 1 mm. B) RT-qPCR analysis for relative HKT1;1 transcript level quantification in pregerminated seedlings. Data are means ± SD (three biological experiments, ∼30 seeds each) and different letters above the bars indicate significantly different values by Tukey's HSD post hoc test (P < 0.01).