PuMYB12 promotes PuSUT4-like expression, while PuPRE6 inhibits the PuSUT4-like expression. A and B) Y1H analysis of PuMYB12 A) or PuPRE6 B) binding to the promoter of PuSUT4-like. Numbers under the panel indicate the serial 10-fold dilutions of samples. C and D). ChIP-PCR showing the binding of PuMYB12 C) or PuPRE6 D) to the PuSUT4-like promoter in vivo. Immunoprecipitated DNA values were normalized to input DNA values. E) PuMYB12 or PuPRE6 effector vectors together with the reporter vector containing the PuSUT4-like promoter were infiltrated into wild Nicotiana tabacum leaves to analyze the regulation of GUS activity. Error bars represent SE obtained from 3 biological replicates. Asterisks indicate significant differences as determined by Student's t-test (**P < 0.01).