AMX treatment impacts the activation state and functions of mature BM neutrophils. CX3CR1^gfp/+ mice were infected with Sp 7 days post-IAV infection and treated with AMX 12 h after Sp infection (IAV/Sp/AMX) or left untreated (IAV/Sp). Mice were sacrificed 12 h post-AMX treatment. (A) The MFIs of CXCR2, CD11b, and CD62L expression on mature BM neutrophils were analyzed by flow cytometry. Representative histograms (top) and mean ± SEM of 1 experiment representative of 3 (lower) are shown (n = 5–6). (B) Total BM cells were incubated with pHrodo E. coli bioparticles and the percentage of mature BM neutrophils positive for E. coli pHrodo (left) as well as the MFI of E.coli pHrodo (right) were quantified by flow cytometry. Data represent pooled results from 3 independent experiments (n = 13) (***P < 0.001; Mann-Whitney). (C) Total BM cells were treated with TBHP (or PBS) and then incubated with CellROX Deep Red Reagent. Results are expressed as mean ± SEM of CellROX MFI of 1 experiment representative of 2 (n = 5–6) (*P < 0.05, **P < 0.01, ***P < 0.001; 2-way analysis of variance).