AMX treatment of infected animals reduces the inflammatory response. C57BL/6J mice were infected with Sp 7 days post-IAV infection and treated with AMX 12 h after Sp infection (IAV/Sp/AMX) or left untreated (IAV/Sp). Lungs and blood were collected at different time points posttreatment. (A) Relative expression of selected genes in the lungs was assessed by real-time quantitative PCR. For each gene, mRNA level is normalized to the reference genes Actb and B2m, and then to the control group IAV/AMX. (B) IL-6 and G-CSF concentrations in the sera were measured by enzyme-linked immunosorbent assay. Data represent pooled results from 2 independent experiments (n = 10) and are expressed as mean ± SEM (*P < 0.05, **P < 0.01 and ***P < 0.001; 2-way analysis of variance test).