Effects of CmNAC100L1 and point mutation of CmCalS1 on its enzyme activity. A Comparison of effects of figleaf gourd and ‘Dongyangshenli’ CmNAC100L1 on CmCalS1 enzyme activity in vitro. CmNAC100L1 was loaded at 3.5 ng and CmCalS1 at 85 ng. B Point mutation increased CmCalS1 activity. C Point mutation decreased CmCalS1 activity.