Analysis of miR172 regulation on AP2L in various ornamentals. (a) Phylogenetic tree analysis of AP2Ls in plants. (b) Prediction of miR172 on AP2L homologs in other ornamental plants. (c) Validation of the miR172-AP2L interaction in different ornamental plants. The upper panels represent the mixed injection of vectors, the middle panels show representative leaf images, and the lower columns represent the statistical analyses of relative luminescence intensities. The relative luminescence intensities were calculated with the value of EV combination as 1, respectively. Data are represented as the mean ± SD of five biological replicates (n = 5). Different letters above the bars indicate a significant difference (P < 0.05, one-way ANOVA).