Effects of cGNAT2 deletion on growth and AcCoA content in Syn7002. Growth curves of WT and ΔcGNAT2 strains under photoautotrophy A), photoheterotrophy (G represents glycerol) B), and high CO2C) conditions. Data represent the mean ± Sd of 3 independent experiments. D to F) CO2 exchange profiles of the WT and ΔcGNAT2 strains under different growth conditions. Cells were suspended in 15 mL of 50 mM HEPES–NaOH buffer (pH 7.5) to final concentration 5.5 μg Chl/mL, and measurements were performed at 20 °C with light intensity of 950 μmol photons/m2/s. Data represent the mean ± Sd of 3 independent experiments. G, H) AcCoA content in WT and ΔcGNAT2 strains under photoautotrophy and photoheterotrophy conditions. I, J) Lysine acetylation in WT and ΔcGNAT2 strains under photoheterotrophy. Densitometry analysis was performed using ImageJ software. Samples were standardized by comparison with Coomassie blue–stained gels. In the boxplots, the bottom and top of the plot represent the lower and upper quartiles, respectively. The box limits inside represent the interquartile range, with the median indicated by a line. The whiskers on both ends represent the minimum and maximum values of the data. Data represent the mean ± Sd of 3 independent experiments. Statistical significance was determined using 2-sample Student's t tests (*P < 0.05; **P < 0.01).
