ET and JA pathway independently regulate thigmomorphogenesis. Photographs (A), flowering times (B), and GA4 levels (C). For (A) to (C), plants were treated with touch as described in Fig. 4. Two hours after the last touch treatment, the aboveground part of each plant was harvested for GA4 quantification. Photos were taken when the plants were 42 d old. Bar = 5 cm. GA, gibberellin; FW, fresh weight. D) Relative transcript levels of GA2ox7 and GA2ox8 in Col-0, dde2, ein2, and ein2 dde2 plants. Twenty-eight-day-old plants were treated with a single touch treatment, and the aboveground part of each plant was harvest 1 h (GA2ox8) and 2 h (GA2ox7) later for RT-qPCR. All data are means ±Se. Different letters indicate statistical differences (1-way ANOVA followed by Duncan's test; P < 0.05, for (B), n = 17; for (C) and (D), n = 5). Statistically significant effect of genotype and treatment was determined by 2-way ANOVA (Supplemental Table S6).
