Saturation curves of SyNadB for L-aspartate and fumarate. A) Saturation curves of SyNadB for L-aspartate under aerobic and anaerobic reactions. For the measurement of SyNadB activity for the anaerobic reaction, the concentration of fumarate was fixed at 2 mM. The coefficients of determination (R2) of curve fitting in the aerobic and anaerobic reactions were 0.97819 and 0.98876, respectively. The χ2 values of curve fitting in the aerobic and anaerobic reactions were 0.00046 and 0.00032, respectively. B) Saturation curve of SyNadB for fumarate in the anaerobic reaction. The concentration of L-aspartate was fixed at 100 mM. The R2 and χ2 of curve fitting were 0.95209 and 0.00151, respectively. The data in Fig. 3 represent the mean ± Sd based on 3 independent measurements.
