USP38 expression is upregulated in pressure overload-induced atrial tissues. (A) Representative western blot images and statistical analysis of the USP38 protein level in left atrial tissues from wild-type mice after AB surgery at 2 and 4 weeks (n = 3). (B) Representative western blot images and statistical analysis of the USP38 protein level in HL-1 cells 48 h after Ang II or PBS stimulation (n = 3). (C) Representative images of immunohistochemical staining and statistical analysis of the USP38 level in left atrial tissues from wild-type mice 0 and 4 weeks after AB surgery (n = 4). Data were calculated by one-way analysis of variance (Tukey’s multiple comparisons test) or Student’s t-test (unpaired, two-tailed, two groups). *P < 0.05; **P < 0.01.