Sequence analysis and luciferase assay confirmed the binding between miR-150-5p and CXCL12 mRNA. And the expressions of CXLC12 mRNA and protein were evidently decreased by the overexpression of miR-150-5p; (A) sequence analysis by TargetScan demonstrated potential binding site between miR-150-5p and CXCL12 mRNA 3′UTR; (B)compared with the transfection of blank control or negative control miRNAs, the transfection of miR-150-5p mimics significantly reduced the fluorescence intensity of mouse primary microglia transfected with vectors carrying wild-type CXCL12 3′UTR; (C) the successful transfection of miR-150-5p mimics was verified by the significant increase of relative expression of miR-150-5p; (D) qRT PCR analysis of CXCL12 mRNA indicated that the overexpression of miR-150-5p evidently inhibited the relative expression of CXCL12 mRNA; (E) western blot assay of CXCL12 protein indicated that the transfection of miR-150-5p mimics inhibited the relative expression of CXCL12 protein.