Higher frequencies of CXCR6⁺DNAM-1^negNKG2A⁺ BM NK cells in BCP-ALL patients. (A) Representative flow cytometry plots showing of CXCR6 + CD69+ BM NK cells in healthy donors (HDs) or BCP-ALL patients. (B) Frequencies of CXCR6⁺CD69⁺ BM NK cells in HDs (n = 10) or BCP-ALL patients at diagnosis according to their risk status: SR (n = 25), IR (n = 13). Data are presented as percentage ± SEM. (C) Degranulation of CD56^dim or CXCR6⁺ BM NK cells after co-incubation of BM mononuclear cells with K562 target cells for 4 h. (D) DNAM-1 expression by BM NK cell subsets in HDs or BCP-ALL patients. (E) Frequencies of DNAM-1^neg CXCR6⁺ NK cells in HDs (n = 10) vs BCP-ALL patients (n = 38). (F) Degranulation of DNAM-1^posCXCR6⁺ vs DNAM-1^negCXCR6⁺ BM NK cells in HDs (n = 4) vs BCP-ALL patients (n = 7). (G, H) Frequencies of DNAM-1/NKG2A subsets in HDs (n = 3) vs BCP-ALL patients (n = 6). Data presented as percentage ± SEM, statistical significance was calculated using the nonparametrical Mann-Whitney test.