PVL-induced NETs do not kill MRSA. (A) Primary human neutrophils were stimulated with PVL (100 nM), PMA (100 nM), or nigericin (30 µM) for 4 h to induce NETs. MRSA was incubated with these NETs for 1 h and colony-forming units was quantified after overnight incubation on TSA plates. Bacterial viability is expressed as a percentage of colony-forming units normalized to MRSA incubated in the absence of NETs. (B) The DNA content of PVL-, PMA-, and nigericin-induced NETs at 4 h was quantified by spectrophotometry. (A) Mean ± SD of 5 independent experiments. *P < 0.05, *** P < 0.001, 1-way analysis of variance. (B) Mean ± SD of 4 independent experiments. ns = not significant.