Single-cell transcriptomic analysis reveals heterogeneity of human thymic iNKT cells. (A) Schematic representation of the experimental pipeline. (B) UMAP plot of scRNA-seq data showing distinct clusters of human thymic iNKT cells. (C) Single-cell heatmap representing the 10 most highly differentially expressed genes in human thymic iNKT cell clusters. Expression for each gene is scaled (z-scored) across single cells. (D, E) Relative expression and normalized counts of SOX4 (D) and KLRB1 (E) in human thymic iNKT cell clusters.