Scatter plots of selection coefficients of BVAL determined under different growth conditions plotted against the selection coefficients in complex medium at 37 °C °C as shown in Fig. 2A. Red circles: outliers from DFE under different conditions, black diamonds: outliers from DFE in complex medium at 37 °C, grey: strains with small effect transfers. A Complex medium including the lag phase at 37 °C. B Complex medium excluding the lag phase at 42 °C. C Defined medium excluding lag phase at 37 °C. D Defined medium with glycerol as only carbon source excluding lag phase at 37 °C.