A Experimental design for functional characterization of the mini“PTS” genes in R. cellulolyticum H10 by genetic disruption, physiological and transcriptomic profiling under different carbon sources, as well as CHIP-Seq and IP-MS. B The number of DEGs across mutants grown with either cellobiose (CB) or carbon mix (CM). All mutants were compared to the parental strain to count DEGs (p < 0.01 and log₂ |FC| > 2, n = 3). C Changes in the number of DEGs in mutants versus the parental strain at 0, 1, 3, 6, 12 h of cellulose incubation experiments (n = 3). D GO enrichment analysis of DEGs in Δcph, ΔccpA, and ΔccpB. Δcph, ΔccpA, and the parent strain after 12 h cellulose incubation were compared. ΔccpB and the parental strain at the mid log phase of growth on cellulose were compared. E Heatmap of transcriptional abundance of select genes in mutants during cellulose incubation. The transcript abundance in each genetic background was normalized to the mean of non-incubated (0 h) conditions. Select genes were shared DEGs in Δcph vs parent and ΔccpA vs parent after 12 h cellulose incubation. The p_perm values indicate whether gene-specific response patterns between mutants and parental strains on cellulose are significantly similar. Genes associated carbohydrate transport and metabolism (Carb. met.) are annotated. Transcriptomic responses of select genes in key gene clusters related to cellulose degradation (F), cellobiose catabolism (G), xylan degradation (H), and a cellulose-responsive TCS (I). Data represents mean ± se (n = 3).