Phylogenetic tree of Wolbachia based on 211 aligned and concatenated BUSCO genes. ML tree based on concatenated sequence alignments of 211 genes from 27 historic and contemporary samples that were either infected with wMel (blue) or wMelCS (red) and wYak as an outgroup. The 8 historic samples that were included in this analysis are highlighted in green. Blue numbers indicate bootstrap values > 75% from 100 rounds of bootstrapping.