Contamination of historic samples with human and Gryllus DNA. Panel A) with stacked barplots showing the frequency of BLAST hits for the 3 most commonly detected genera based on BLAST searches of 100,000 mt reads against the NCBI nt database. Panel B) shows a phylogeny based on the Wolbachia-specific wsp locus for Wolbachia samples from supergroup B, an NCBI GenBank sequence from a Wolbachia variant specific to Gryllus bimaculatus and 2 Wolbachia sequences from the historic samples 19SL3 and 19SL22.