Figure 4.
Representative figures of microscopic TDP-43 findings. Amygdala slides stained with antibodies against phospho-TDP-43 showed star-shaped TDP-43-immunoreactive inclusions in medial subpial regions (A) and basolateral white matter (B) and subependymal regions (thick arrow in C). Additionally, dot-like neuropil (A) and a long thread-like neurite (thin arrow in C) are seen. Different morphologies of star-shaped inclusions are shown: star-shaped with long, branching processes (D, E), with lace-like processes (F), with granular appearance (G, H), or with crown-like (I) or thorn-like (J) appearance. An occasional coiled body-like inclusion is shown (K). Superficial thin, thread-like neurites are commonly seen (L). In the hippocampus, a variety of neurites from thin to thick and short to long are seen in the fimbria (M), as well as the subiculum and entorhinal cortex (not shown). Using antibodies against N-terminal TDP-43 (N), star-shaped TDP-43 inclusions with long processes are also seen (thick arrow), although many of the inclusions are more granular in appearance (thin arrows and inset in N). Using antibodies against the C-terminal region (O), only a few granular star-shaped inclusions are seen (thick arrow) and some superficial neurites (thin arrow). However, some comma-shaped thick neurites (pink arrowheads in P) and neuronal cytoplasmic inclusions (pink arrowheads and inset in Q) are additionally seen. Scale bars: A-C, M-Q = 40 μm, D-K and all insets = 30 μm.

Representative figures of microscopic TDP-43 findings. Amygdala slides stained with antibodies against phospho-TDP-43 showed star-shaped TDP-43-immunoreactive inclusions in medial subpial regions (A) and basolateral white matter (B) and subependymal regions (thick arrow in C). Additionally, dot-like neuropil (A) and a long thread-like neurite (thin arrow in C) are seen. Different morphologies of star-shaped inclusions are shown: star-shaped with long, branching processes (D, E), with lace-like processes (F), with granular appearance (G, H), or with crown-like (I) or thorn-like (J) appearance. An occasional coiled body-like inclusion is shown (K). Superficial thin, thread-like neurites are commonly seen (L). In the hippocampus, a variety of neurites from thin to thick and short to long are seen in the fimbria (M), as well as the subiculum and entorhinal cortex (not shown). Using antibodies against N-terminal TDP-43 (N), star-shaped TDP-43 inclusions with long processes are also seen (thick arrow), although many of the inclusions are more granular in appearance (thin arrows and inset in N). Using antibodies against the C-terminal region (O), only a few granular star-shaped inclusions are seen (thick arrow) and some superficial neurites (thin arrow). However, some comma-shaped thick neurites (pink arrowheads in P) and neuronal cytoplasmic inclusions (pink arrowheads and inset in Q) are additionally seen. Scale bars: A-C, M-Q = 40 μm, D-K and all insets = 30 μm.

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