Figure 6.
Thin-layer chromatography of the [14C]chloramphenicol (Cm) CAT assay products for determination of the relative strengths of group IIC-attC intron promoters (Pout). The TLC plate was exposed to a Kodak BioMax MR film to obtain this image. CAT activity was assayed as described in ‘Materials and Methods’ section. The pKK232 clones used for this assay are described in Table 1. pLQ872 and pLQ876 contain the weak and strong versions of integron Pc promoters (respectively) cloned into pKK232-8 (1). (a) indicates the origin; (b) indicates non-acetylated Cm; (c) indicates 1-acetoxy-Cm; (d) indicates 3-acetoxy-Cm.