MdHDA6 directly interacts with MdABI5. A) Truncation schematic diagram of MdHDA6 and MdABI5. B) Yeast 2-hybrid (Y2H) assays of MdHDA6 and MdABI5, as well as their truncated proteins. C) BiFC assay to verify the interaction of MdHDA6 and MdABI5. Bars = 100 μm. D) Split-LUC assay was applied to verify the interaction of MdHDA6 and MdABI5. E) Co-IP assay was used to validate the interaction of MdHDA6 and MdABI5. MdHDA6 fused with GFP and MdABI5 with MYC (Myelocytomatosis) tags were transiently expressed in tobacco. The anti-MYC antibody was used for immunoprecipitation and the anti-MYC and anti-GFP antibodies were used for western blot assay.