Fig. 1.
Phylogeny of Heartland and Bourbon viruses. Alignments were performed using MAFFT 1.3.7 in Geneious Prime 2022.2.2 and maximum likelihood trees were created using RAxML with a GTR gamma model and 500 bootstraps. Node values represent bootstrap support in unrooted trees. (A) All available sequences of L segments for Heartland virus (HRTV) are shown together with representative strains of closely related viruses including Guertu virus, Dabie bandavirus, Kismayo virus, Bhanja virus, Lone Star virus, and Hunter Island virus. (B) All available sequences of PB2 genes (segment 1) for Bourbon virus (BRBV) are shown together with representative strains of closely related viruses including Dhori virus, Batken virus, Oz virus, Thogoto virus, Aransas Bay virus, and Influenza A virus.