Global analysis of ortholog expression among diatom strains Aitchison PCoA of conserved orthogroups that had a prevalence of a) 10 counts per sample (N = 2,933 orthogroups) and b) 300 counts per sample (N = 229 orthogroups). c) Hierarchical clustering using Aitchison distance of conserved orthogroup expression with at least 300 counts per sample. Circles represent ancestral controls, and triangles represent bottlenecked strains followed by FS recovery (i.e. full selected). In c), strain labels are followed by either “C” (e.g. TP-3367-C) to designate a control population or a number (e.g. TP-3367-4) to designate the replicate population number for that strain. The colored circles within each strain grouping represent 3 SD from the centroid.