Distribution and clustering of the cleavage site and non-cleavage sites based on the sequence scores selected by the IFS algorithm for six proteases: (A) caspase-1, (B) caspase-3, (C) caspase-6, (D) MMP-2, (E) MMP-3 and (F) Granzyme B. For each protease, the samples are clustered into two groups using the K-means algorithm, and clusters are colour-coded. The cleavage and non-cleavage sites are presented by different markers, where dots are for the cleavage sites and ‘×’ for the non-cleavage sites. The inset bar charts show the fractions of cleavage versus non-cleavage sites in each cluster.