Evaluation of eccDNA detection performance of CReSIL and its comparison with the other tools. A) Frequency polygon plot showing the size distribution of simulated eccDNA of true positive (TP, orange line) and true negative (TN, green line) datasets. B) Donut plots showing the percent distribution of the number of chromosomal regions of the individual simulated eccDNA datasets. C) Bar plots showing the number of simulated eccDNA that contains CTC reads (magenta) and non-CTC reads (dark blue) across different sequencing depths. D) Stacked bar plots showing the number of eccDNA detected for the true positive dataset by different tools. The eccDNA detection results are classified into five categories; CircularT (red) = circular sequences with 95% reciprocal overlaps and 90% identity with the simulated eccDNA sequences, CircularF (orange) = circular sequences without the criteria, LinearT (dark gray) = linear sequences with the criteria, LinearF (light gray) = linear sequences without the criteria, and not detected (black) = the tool cannot detect; the number of true positive eccDNA (blue dashed line). E) Stacked bar plots presenting the number of eccDNA detected for the true negative dataset. See the color code for panel D, the number of true negative eccDNA (blue dashed line). F) Point and line plots showing the performance (F1 score) of eccDNA detection of the individual tools across different sequencing depths.