Selection at PIMT genes. (A) McDonald–Kreitman test of PIMT genes. McDonald–Kreitman test and α estimation (McDonald and Kreitman 1991; Smith and Eyre-Walker 2002) for each pair of closest PIMT orthologs between P. vanderplanki and P. pembai, calculated from P. vanderplanki—P. pembai divergence and P. vanderplanki polymorphism. Only those comparisons with at least 5 SNPs in the corresponding P. vanderplanki population are shown. (B) Identification of sites in PIMTs genes under positive selection. The only residue with BEB score >50 is shown in red. Gray boxes indicate the two short regions specific to the PIMTs of Polypedilum sp.