Transcriptomic changes in bulk uterine natural killer (uNK) cells following hormonal stimulation. (A) Heat map showing differentially expressed genes based on bulk RNAseq of sort-purified uNK cells. Upregulated genes expressed in red with downregulation in blue. (B) Volcano plot of protein-coding genes examined via RNAseq. (C) Ingenuity pathway analysis of protein-coding genes showing top canonical pathways, upstream regulators, and biological function, and networks. In bold are pathways/genes/functions associated with immune cell trafficking and movement, and inflammation associated pathways, genes, and functions are italicized. 1: Tkachenko et al., 2011; 2: Sharfe et al., 2002; 3: Leong et al., 2015; 4: Wang et al., 2016; 5: Horwood et al., 2012; 6: Barter et al, 2004; 7: Guezguez et al., 2007; 8: Shannon and Mace, 2021; 9: Chatterjee et al., 2012; 10: Fontana et al., 2015. VTCN1, V-set domain containing T-cell activation inhibitor 1; CST1, cystatin SN; NTN4, netrin 4; CHIT1, chitinase 1; LDHC, lactate dehydrogenase C; CPB1, carboxypeptidase B1.