Raw flow cytometry gating strategy used to identify basic immune populations. Blood and endometrial (Endo) profiles reveal tissue-specific effects on immune composition. Data shown are from matched blood and endometrial biopsy from the same patient cycling naturally. Samples were processed and stained with the identical antibody cocktails at the same time. (A) Gating scheme for live, singlet, and CD45+ cells. Among CD45+ cells, we show T cells (CD3+CD56−), natural killer T-like (NKT-like) cells (CD3+CD56+), NK cells (CD56+CD3-), B cells (CD19+CD3−), bulk CD14+ monocytes (blood), and bulk CD14+ monocytes/macrophages (endometrium). (B) Subsetting of CD45+CD3+ T cells by expression of CD4 and CD8. CD4+ T cells are rare in the endometrium, while CD4/CD8 double-negative T cells emerge in the endometrium. Numbers on plot denote percent of cells within indicated gates.