Subcellular location and colocalization of AsNIP43 and NopP in N. benthamiana cells. A and B) Confocal microscopy images of the N. benthamiana leaf cells co-expressing AsNIP43-GFP with the plasma membrane marker CERK1-DsRed, and the ER marker HDEL-mCherry. C and D) Confocal microscopy images of N. benthamiana cells co-expressing NopP-GFP with the plasma membrane marker, or the ER marker. E) Localization of free eGFP and DsRed from control vectors. F) Co-localization of AsNIP43-GFP and NopP-DsRed. From left to right, GFP channel, DsRed channel, bright field channel, and overlay. Scale bars, 40 μm A, B, D to F), 25 μm C).