Fig. 3
Nucleus deformation in the sun2 KO line. (A) Shape of the prophase nucleus in protonemal apical cells 14 min before NEBD. Green; microtubules. Magenta; chromosomes. Bar, 5 µm. (B) Circularity of the nucleus in protonemal apical cells 14 min before NEBD. Mean ± SEM (from left to right): 0.769 ± 0.0107 (n = 17), 0.947 ± 0.00711 (n = 10) and 0.769 ± 0.0148 (n = 12). P-values based on two-sided Tukey’s multiple comparison test; P < 0.0000001 (subapical cells: control vs. sun2 KO) and P < 0.0000001 (subapical cells: sun2 KO vs. sun2 KO/SUN2 [full-length]-mCerulean). (C) Shape of the nucleus in interphase in subapical cells. Green; microtubules. Magenta; chromosomes. Bar, 5 µm. (D) Circularity of the nucleus in interphase subapical cells. Mean ± SEM (from left to right): 0.658 ± 0.0201 (n = 20), 0.833 ± 0.0141 (n = 20) and 0.662 ± 0.0211 (n = 20). P-values based on the two-sided Steel–Dwass test; P < 0.00001 (control vs. sun2 KO) and P < 0.00001 (sun2 KO vs. sun2 KO/SUN2 [full-length]-mCerulean).

Nucleus deformation in the sun2 KO line. (A) Shape of the prophase nucleus in protonemal apical cells 14 min before NEBD. Green; microtubules. Magenta; chromosomes. Bar, 5 µm. (B) Circularity of the nucleus in protonemal apical cells 14 min before NEBD. Mean ± SEM (from left to right): 0.769 ± 0.0107 (n = 17), 0.947 ± 0.00711 (n = 10) and 0.769 ± 0.0148 (n = 12). P-values based on two-sided Tukey’s multiple comparison test; P < 0.0000001 (subapical cells: control vs. sun2 KO) and P < 0.0000001 (subapical cells: sun2 KO vs. sun2 KO/SUN2 [full-length]-mCerulean). (C) Shape of the nucleus in interphase in subapical cells. Green; microtubules. Magenta; chromosomes. Bar, 5 µm. (D) Circularity of the nucleus in interphase subapical cells. Mean ± SEM (from left to right): 0.658 ± 0.0201 (n = 20), 0.833 ± 0.0141 (n = 20) and 0.662 ± 0.0211 (n = 20). P-values based on the two-sided Steel–Dwass test; P < 0.00001 (control vs. sun2 KO) and P < 0.00001 (sun2 KO vs. sun2 KO/SUN2 [full-length]-mCerulean).

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