Physiological changes and gene expression analysis of banana peels in response to low temperature. A) Browning spots appear on banana peels at low temperature (LT). Fruits were kept at 24 °C (room temperature [RT]) or 6 °C for 0, 1, or 3 d. The fruit images were digitally extracted for comparison. Scale bars = 2 cm. B) Lightness of banana peels at low temperature after 0, 1, or 3 d at 6 °C. Data are shown as means ± Se (standard error, n ≥ 3); asterisks represent significance between 2 treatments (t-test, *P < 0.05; **P < 0.01). C) Time course of gene expression for 3 CBFs, LOX6, PPO1, and POD2, at RT and LT. Data are shown as means ± Se (standard error, n = 3). D) PCA of RNA-seq data. Each circle represents 1 sample. E) DEGs in banana peels in response to low temperature. Venn diagram showing the number of common and time-specific DEGs. F) Heatmap of the RNA-seq and DHS data sets generated by k-means clustering analysis. PC1 represents a cluster of linear equation. RNA-seq and DNase-seq values were normalized. Representative genes are listed to the left. The patterns of PC1 are shown in the middle. Selected GO terms are listed to the right.