IFITM3 expression before and during DAA treatment. IFITM3 mRNA levels were measured by qPCR in individual hepatocytes before and after DAA initiation in all participants and are shown (A) in aggregate and (B) individually for all participants. The individual measurements per cell are represented by the filled black points. The boxplots represent the 25th and 75th percentiles (bottom and top edge of the box), the median (thick line across the box), whiskers extending from the edge of the box to the smallest (bottom) or largest (top) value no further than 1.5 times the interquartile range (the height of the box) from the box’s edges. Abbreviations: DAA, direct-acting antiviral; qPCR, quantitative polymerase chain reaction; SV, sofosbuvir-velpatasvir; SVV, sofosbuvir-velpatasvir-voxilaprevir.