Comparison of intracellular HCV RNA in single hepatocytes. scLCM was used to isolate 300–400 hepatocytes per liver biopsy before and 4 or 7 days after DAA initiation. qPCR was used to measure HCV RNA in each hepatocyte and is shown (A) in aggregate across all 10 participants or (B) individually. Note that here we only show the levels of HCV RNA in infected cells. Thus, participant 7, for whom we could not find any HCV-infected cells after DAA initiation, is not shown in (B). Intracellular HCV quantities are reported as IU/cell; each IU corresponds to approximately 1.96 copies/cell. The individual measurements per cell are represented by filled black points. The boxplots represent the 25th and 75th percentiles (bottom and top edge of the box), the median (thick line across the box), whiskers extending from the edge of the box to the smallest (bottom) or largest (top) value no further than 1.5 times the interquartile range (the height of the box) from the box’s edges. Abbreviations: DAA, direct-acting antiviral; HCV, hepatitis C virus; qPCR, quantitative polymerase chain reaction; scLCM, single-cell laser capture microdissection; SV, sofosbuvir-velpatasvir; SVV, sofosbuvir-velpatasvir-voxilaprevir.