CYP37 did not affect the assembly and accumulation of thylakoid membrane proteins. A) Blue native/PAGE and subsequent analysis of thylakoid membrane complexes in 2D SDS/PAGE. Three-wk-old WT and cyp37-1 mutant plants grown in GL conditions (16 h light/8 h dark, 90 μmol·m−2·s−1) were transferred to HL conditions (10 h light/14 h dark, 350 μmol·m−2·s−1) and treated for 5 d. 1, NDH (NADPH dehydrogenase complexes); 2, PSII SC (PSII supercomplexes); 3, PSI-M (PSI monomers), PSII-D (PSII dimers), PSII-M (PSII monomers), and LHC-T (LHC trimers); 4, PSI-M (PSI monomers), CF1 (a catalytic component of ATP synthase); 5, PSII-M (PSII monomers), Cyt b6/f (cytochrome b6/f complex); 6, LHCII assembly; 7, LHCII-T. B) Five micrograms of proteins (based on chlorophyll quantification) from isolated chloroplasts were separated by 12% SDS‒PAGE and immunoblotted with anti-PetA (photosynthetic electron transfer A), anti-PetB (photosynthetic electron transfer B), anti-PetC (photosynthetic electron transfer C), anti-PsaD (photosystem I subunit D), anti-PsaF (photosystem I subunit F), anti-D1 (PSII reaction center protein A), anti-D2 (photosystem II reaction center protein B), anti-psbO (photosystem II subunit O), and anti-ATPa (ATP synthase subunit a) antibodies. The sample containing 5 μg chlorophyll was labeled 1 unit (U). U, 1/2 U, and 1/4 U of protein samples from WT, as well as 1 U protein samples from cyp37-1 mutant, were loaded. Three-wk-old WT and cyp37-1 mutant plants grown in GL (16 h light/8 h dark, 90 μmol·m−2·s−1) were transferred to HL conditions (10 h light/14 h dark, 350 μmol·m−2·s−1) and treated for 7 d.
