CsSCL2 and CsSCL3 interacted with CsClot. A) Interaction of CsSCL2G, CsSCL3G with CsClot verified by Y2H assays. BD-CsSCL2G and BD-CsSCL3G were respectively cotransformed with AD-CsClot in AH109 yeast strain to verify the interaction. Cotransformation of BD-p53 and AD-RecT was used as the positive control. Cotransformation of BD-Laminc and AD-RecT was used as the negative control. EV, empty vector; SD/-T/-L, SD medium without Trp and Leu; SD/-T/-L/-H/-A, SD medium without Trp, Leu, His, and Ade; SD/-T/-L/-H/-A/+X-α-gal, SD medium without Trp, Leu, His, Ade, and with X-α-gal. B) The interactions were detected between CsSCL2, CsSCL2G, CsSCL3, CsSCL3G, and CsClot by split firefly luciferase complementation imaging (LCI) assays. CsClot-Nluc and Cluc-CsSCLs (i.e. CsSCL2, CsSCL2G, CsSCL3, and CsSCL3G) were respectively coinjected into N. benthamiana leaves to observe the luciferase signal. Nluc + Cluc, CsSCLs-Nluc + Cluc, and Nluc + Cluc-CsClot were used as negative controls. C) Co-IP analyses show that CsSCL2 and CsSCL3 interact with CsClot. The bands in input assay indicated proteins expressed in N. benthamiana leaves. Co-IP was performed using anti-GFP Nanobody Magarose Beads, and Western blot were performed using anti-MYC (α-MYC) and anti-GFP (α-GFP). Cotransformation of the GFP empty vector (GFP) and MYC-rSCL2, MYC-rSCL3 were used as negative controls.