Layer specificity of microdissected neurons. (A) Representative tumor mass and peritumoral neurons in the primary visual cortex. The box indicates the ROI for the laser-capture microdissection (LCM). Scale bar, 100 µm. (B) Representative images of LCM: Labeled neurons were selected (left), removed by the laser (middle), and collected in the adhesive cap (right). (C) Gene-expression analysis of Slc17a7 and Slc32a1 in all samples detected by quantitative real-time PCR. Slc17a7 encoded for vGlut1 protein, specific for glutamatergic neurons; Slc32a1 encoded for the protein vGat specific for inhibitory neurons. The mean ± sem is shown for each group. (D) Layer organization of primary visual cortex and corresponding layer-specific genes included in the panel: Cux1 is a marker of the layers II-III, cTip2 of the layers V-VI, and FoxP2 of the inner layer VI. Scale bar 100μm. Gene-expression values were normalized for reference genes and expressed as an increase or decrease relative to the inter-run calibrator (Hellemans et al., 2008; Rieu and Powers, 2009). The mean ± sem is shown for each group.