Inhibition of nuclear phosphorylated-STAT3 expression in the area postrema during CIA by BR. (A) Representative images of phosphorylated-STAT3 immunostaining with nuclear staining (DAPI) in the area postrema on PID 35 in the vehicle (left panels) and BR group (right panels). Yellow dashed lines indicate the boundaries of the area postrema. Boxed areas are shown at higher magnification on the lower (a) to (f). (B–D) Results of quantitative analysis for pSTAT3 intensity in the intranuclear area (B) and extranuclear area (C) of the area postrema (Veh, n = 11 mice; CIA, n = 10 mice). Normalized intranuclear pSTAT3 intensity to extranuclear pSTAT3 intensity (D) is significantly lower in the BR group. BR: baricitinib; DAPI: 4′,6-diamidino-2-phenylindole; PID: post immunization day; STAT3: signal transducers and activators of transcription; Veh: vehicle