Mass spectrometry analysis of proteins secreted basolaterally by hormone- and PGE2-treated human endometrial epithelial organoids. Organoids were seeded in Matrigel and grown for 4 days in expansion media, followed by treatment with vehicle as a control or estrogen (E2) for 2 days. Next, the ExM was replaced with base media, and organoids were treated for 6 days with either vehicle as a control, E2, E2 + medroxyprogesterone acetate (MPA), MPA alone, or MPA + prostaglandin E2 (PGE2) for 6 days. On Day 12, proteins in the base media surrounding the organoid were determined by mass spectrometry analysis. (A) ShinyGO visualization of enriched biological processes for genes identified in organoid conditioned media. (B) Integration of proteins that were increased or decreased for all treatments compared to control. (C) REVIGO visualization of enriched biological processes for proteins increased or decreased by E2 and E2 + MPA treatment compared to control.