Single-cell RNA-seq analyses of human and murine embryonic hearts. (A) The tSNE analysis using the single-cell RNA-seq dataset, which was obtained from human embryonic hearts (4.5–10 weeks of foetal ages),¹⁶ segregated 458 individual cardiac cells into 10 molecularly distinct clusters, including a cono-ventricular region-specific heart progenitor (CVP; Cluster #1). (B) A heatmap image showing the representative differential expression genes in each of the 10 clusters in (A). (C) Feature plots of the SHF/OFT marker (CVP-enriched) genes as well as the pan-cardiac/FHF/developing CM marker genes with STRA6 on the tSNE plots in (A). (D) Violin plots of the same genes as in (C), in the segregated 10 clusters of the human embryonic heart-derived single cells. (E) The top 25 genes correlated with the expression of the CVP-specific gene LGR5 in single-cell RNA-seq data of human embryonic hearts. Corrected P-value for each gene was calculated by Guilt-by-Association and correlation analysis.¹⁴ (F) The tSNE analysis segregated a total of 2079 single cardiac cells, obtained from embryonic hearts (E9.5, E10.5, and E14.5) of the Mesp1⁺ lineage tracing mice (Mesp1^Cre/+; Rosa26^tdTomato), into 16 clusters including FHF progenitors (Cluster #1) and SHF progenitors (Cluster #2). (G) Feature plots of the SHF/pan-cardiac/FHF/developing CM marker genes with STRA6 on the tSNE plots in (F). (H) Violin plots of the same genes as in (G), in the segregated 16 clusters of the murine embryonic heart-derived single cells.