Uses of ACT using signal tracks from various sources. Signal around all TSSs is aggregated to give an average signal profile, for example of Baf155 binding around TSSs (Encode Project) (aggregation). Figure made in Excel (correlation). Multiple signal tracks are correlated to show which tracks are more or less related to each other. In the selected example, a heatmap of the SNP track correlation between four individuals (dbSNP) leads to a dendogram of their phylogenetic relationship. Figure made using Web ACT. Each additional signal track increases the number of base pairs covered (saturation). When the addition of signal tracks is considered in all possible combinations, the average increase in coverage, with error bars, can be visualized by a saturation plot. In the example, data are taken from individuals from dbSNP [with additional genomes from Ahn et al. (2009), Bentley et al. (2008), Drmanac et al. (2010), Kim et al. (2009)]. In each box plot, the top and bottom pink bars correspond to the maximum and minimum normal values, the top edge, middle line and bottom edge of the box correspond to the top 25 percentile, median and bottom 25 percentile, the black dot is the mean, and red circles are outliers. Figure made using ACT downloadable saturation program.
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