The AceGGP3 expression is induced by cold stress. A–B) Gene ontology (GO) analysis of significantly up-regulated A) and down-regulated B) genes in kiwifruit calli at RT (room temperature) and COLD (4 °C) for 6 h. C) Expression profiles of 21 genes of L-galactose pathway and 3 transcription factors in kiwifruit under RT and COLD. Log-transformed expression values range from 0 to 1. D) RT-qPCR analysis of AceGGP3 expression of kiwifruit (A. eriantha) calli treated at 4 °C for 0 h, 2 h, 6 h, 12 h, 1 d, and 3 d. E) Relative AsA content of kiwifruit calli in D). AsA content of calli in 0 h was taken as 5 for normalization. F) Relative GUS activity and histochemical staining of the AceGGP3 promoter expression construct AceGGP3::GUS in transgenic kiwifruit calli at 25 °C (control) and 4 °C (cold stress) for 9 h. Error bars denote the standard deviation (± SD), n = 3. Significant differences were detected by t-test (*P < 0.05; **P < 0.01; ***P < 0.001).